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KMID : 0379120080360020178
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Korean Journal of Mycology
2008 Volume.36 No. 2 p.178 ~ p.182
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Screening of Inhibitors of Extracellular Serine Protease of Acanthamoeba castellanii from Mushroom Extracts
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Lee Sung-Eun
Choi Mi-Young
Seo Sung-Yum
Sancheti Sandesh
Sancheti Shruti
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Abstract
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Although the number of patients with Acanthamoeba keratitis has increased dramatically since the widespread use of contact lens, it is still very hard to cure the disease. The proteases from the Acanthamoeba were reported to play important role in the pathogenesis of keratitis. In this study, the inhibitors for extracellular serine proteases of A. castellanii were screened from the extracts of 230 mushroom samples collected from various regions of Korea. The mushrooms were extracted with methanol and water (). Filtered and concentrated extracts (0.3 mg/ml) were preincubated with proteases before addition of peptide substrate N-succinyl-ala-ala-pro-phe p-anilide. The selected extracts showing strong inhibitory effects were characterized. Although inhibition with single extract was not so high enough, the complete inhibition was achieved with combination of two extracts. The selected extract showed little effect on other serine proteases such as thrombin (human and bovine) and on general protease such as protease K.
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KEYWORD
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Acanthamoeba castellanii, Inhibitor, Keratitis Mushrooms, Protease
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